{"id":5900,"date":"2020-05-11T09:34:43","date_gmt":"2020-05-11T07:34:43","guid":{"rendered":"https:\/\/sfbd.fr\/2020\/05\/11\/recrutement-dun%c2%b7e-assistant%c2%b7e-ingenieur%c2%b7e-septembre-2020-pour-12-mois\/"},"modified":"2020-06-02T18:26:14","modified_gmt":"2020-06-02T16:26:14","slug":"recrutement-dun%c2%b7e-assistant%c2%b7e-ingenieur%c2%b7e-septembre-2020-pour-12-mois","status":"publish","type":"post","link":"https:\/\/sfbd.fr\/en\/2020\/05\/11\/recrutement-dun%c2%b7e-assistant%c2%b7e-ingenieur%c2%b7e-septembre-2020-pour-12-mois\/","title":{"rendered":"Recruitment of an Assistant Engineer – September 2020 for 12 months"},"content":{"rendered":"

[vc_row][vc_column][vc_column_text]<\/p>\r\n

– article in French –<\/em><\/p>\r\n\r\n

 <\/p>\r\n\r\n

Institut de G\u00e9nomique Fonctionnelle de Lyon<\/strong><\/p>\r\n\r\n

 <\/p>\r\n\r\n

(ENS de Lyon\/CNRS\/UCBL)<\/strong><\/p>\r\n\r\n

 <\/p>\r\n\r\n

Equipe \u201cPhysiologie Int<\/strong>\u00e9grative des Interactions H\u00f4te-Microbes\u201d<\/strong><\/p>\r\n\r\n

 <\/p>\r\n\r\n

Contact : Cathy Ramos<\/strong> (cathy.ramos@ens-lyon.fr)<\/p>\r\n\r\n

 <\/p>\r\n\r\n

<\/p>\r\n

\u00a0<\/p>\r\n

<\/p>\r\n

Les projets de recherche de l\u2019\u00e9quipe \u00a8Physiologie Int<\/strong>\u00e9grative des Interactions H\u00f4te-Microbes<\/strong>\u00a8 install\u00e9e \u00e0 l\u2019IGFL et dirig\u00e9e par le Dr F. Leulier (Gerland, Lyon) visent \u00e0 \u00e9lucider les m\u00e9canismes cellulaires r\u00e9gissant les interactions b\u00e9n\u00e9fiques entre la flore microbienne intestinale et son h\u00f4te, par des approches de g\u00e9n\u00e9tique, de microbiologie, de biologie mol\u00e9culaire et cellulaire. Ainsi, l\u2019\u00e9quipe utilise comme mod\u00e8le d\u2019\u00e9tude principal l\u2019association entre l\u2019esp\u00e8ce bact\u00e9rienne Lactobacillus plantarum<\/em> et l\u2019insecte Drosophila melanogaster<\/em>.<\/p>\r\n

<\/p>\r\n

Missions<\/strong>
Le projet auquel le\/la collaborateur\u00b7trice prendra part au sein de l\u2019\u00e9quipe porte sur la r\u00e9alisation d\u2019analyses fonctionnelles visant \u00e0 identifier les m\u00e9canismes cellulaires de croissance syst\u00e9mique et tissulaire chez la larve de drosophile. Il ou elle participera au maintien des stocks drosophiles, \u00e0 la g\u00e9n\u00e9ration de lign\u00e9es ax\u00e9niques, \u00e0 la construction de nouvelles lign\u00e9es de drosophile (transg\u00e9niques ou mutantes), \u00e0 la r\u00e9alisation et au suivi de croisements de lign\u00e9es visant \u00e0 activer ou \u00e9teindre l\u2019expression de g\u00e8nes d\u2019int\u00e9r\u00eat ainsi qu\u2019au suivi ph\u00e9notypique des individus g\u00e9n\u00e9r\u00e9s (macroscopique, cellulaire et mol\u00e9culaire).<\/p>\r\n

<\/p>\r\n

Activit\u00e9s<\/strong> –
L\u2019AI devra g\u00e9n\u00e9rer et utiliser les animaux issus des croisements g\u00e9n\u00e9tiques pour les exp\u00e9riences de biologie cellulaire (immunomarquages et observation en microscopie \u00e9pifluorescence et microscopie confocale) et mol\u00e9culaire (extractions d\u2019ADN et\/ou d\u2019ARN \u00e0 partir de diff\u00e9rents organes de larves de drosophile puis tests PCR ou RT-PCR). Bien que les protocoles soient d\u00e9j\u00e0 mis en place au sein du laboratoire, l\u2019AI pourrait \u00eatre amen\u00e9 \u00e0 optimiser certains d\u2019entre eux, notamment les protocoles d\u2019immunomarquages.<\/p>\r\n

<\/p>\r\n


Comp\u00e9tences<\/strong><\/p>\r\n

<\/p>\r\n

– Niveau de formation requis : DUT, BTS, Bac +2 en Biologie<\/p>\r\n

<\/p>\r\n

– Connaissances solides en Biologie cellulaire et mol\u00e9culaire et en g\u00e9n\u00e9tique.<\/p>\r\n

<\/p>\r\n

– Exp\u00e9rience avec utilisation de lign\u00e9es de drosophiles souhait\u00e9e.<\/p>\r\n

<\/p>\r\n

– Maitrise des techniques d\u2019immunomarquages et de microscopie (\u00e9pifluorescence et confocale)<\/p>\r\n

<\/p>\r\n

– Maitrise des techniques de biologie mol\u00e9culaire (extraction et manipulation d\u2019acides nucl\u00e9iques, PCR)<\/p>\r\n

<\/p>\r\n

– Motivation, Autonomie,\u00a0 organisation, observation, rigueur technique, fiabilit\u00e9, respect des bonnes pratiques de laboratoire (r\u00e8gles d\u2019hygi\u00e8ne et de s\u00e9curit\u00e9, gestion des d\u00e9chets).<\/p>\r\n

<\/p>\r\n

– Savoir rassembler et mettre en forme les protocoles et r\u00e9sultats de ses exp\u00e9riences, les pr\u00e9senter clairement.<\/p>\r\n

<\/p>\r\n

– Ma\u00eetrise des outils\/logiciels\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 classiques\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 de\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 bureautique\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 (Excel,\u00a0\u00a0\u00a0\u00a0 Word, PowerPoint)<\/p>\r\n

<\/p>\r\n

– Respect des r\u00e8gles et des proc\u00e9dures, sens pratique d\u00e9velopp\u00e9.<\/p>\r\n

<\/p>\r\n

– Ma\u00eetrise de l\u2019anglais oral souhait\u00e9e.<\/p>\r\n

<\/p>\r\n


Contexte de travail<\/strong>
Le CDD est de 12 mois (temps plein) au sein de l\u2019IGFL \u00e0 partir de Septembre 2020 au sein de l\u2019\u00e9quipe de Physiologie Int\u00e9grative des Interaction H\u00f4te-Microbes dirig\u00e9e par le Dr F. Leulier.<\/p>\r\n

<\/p>\r\n

Pri\u00e8re de prendre contact au plus vite aupr\u00e8s de Cathy Ramos (cathy.ramos@ens-lyon.fr) uniquement par email (stipuler \u00ab Poste AI Genet et BioCell \u00bb dans l\u2019objet).\u00a0 Joindre CV, lettre de motivation et les coordonn\u00e9es de deux r\u00e9f\u00e9rences.<\/strong><\/p>\r\n

<\/p>\r\n


Informations compl\u00e9mentaires<\/strong>
En raison du contexte de pand\u00e9mie Covid19, la date du recrutement pourra \u00eatre report\u00e9e.<\/p>\r\n

<\/p>\r\n

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